Effects of natural and chemically synthesized furanones on quorum sensing in Chromobacterium violaceum

BACKGROUND: Cell to cell signaling systems in Gram-negative bacteria rely on small diffusible molecules such as the N-acylhomoserine lactones (AHL). These compounds are involved in the production of antibiotics, exoenzymes, virulence factors and biofilm formation. They belong to the class of furanone derivatives which are frequently found in nature as pheromones, flavor compounds or secondary metabolites. To obtain more information on the relation between molecular structure and quorum sensing, we tested a variety of natural and chemically synthesized furanones for their ability to interfere with the quorum sensing mechanism using a quantitative bioassay with Chromobacterium violaceum CV026 for antagonistic and agonistic action. We were looking at the following questions: 1. Do these compounds affect growth? 2) Do these compounds activate the quorum sensing system of C. violaceum CV026? 3) Do these compounds inhibit violacein formation induced by the addition of the natural inducer N-hexanoylhomoserine lactone (HHL)? 4) Do these compounds enhance violacein formation in presence of HHL? RESULTS: The naturally produced N-acylhomoserine lactones showed a strong non-linear concentration dependent influence on violacein production in C. violaceum with a maximum at 3.7*10(-8 )M with HHL. Apart from the N-acylhomoserine lactones only one furanone (emoxyfurane) was found to simulate N-acylhomoserine lactone activity and induce violacein formation. The most effective substances acting negatively both on growth and quorum sensing were analogs and intermediates in synthesis of the butenolides from Streptomyces antibioticus. CONCLUSION: As the regulation of many bacterial processes is governed by quorum sensing systems, the finding of natural and synthetic furanones acting as agonists or antagonists suggests an interesting tool to control and handle detrimental AHL induced effects. Some effects are due to general toxicity; others are explained by a competitive interaction for LuxR proteins. For further experiments it is important to be aware of the fact that quorum sensing active compounds have non-linear effects. Inducers can act as inhibitors and inhibitors might be able to activate or enhance the quorum sensing system depending on chemical structure and concentration levels

Nanorice Particles: Hybrid Plasmonic Nanostructures

A new hybrid nanoparticle, i.e., a nanorice particle, which combines the intense local fields of nanorods with the highly tunable plasmon resonances of nanoshells, is described herein. This geometry possesses far greater structural tunability than previous nanoparticle geometries, along with much larger local field enhancements and far greater sensitivity as a surface plasmon resonance (SPR) nanosensor than presently known dielectric-conductive material nanostructures. In an embodiment, a nanoparticle comprises a prolate spheroid-shaped core having a first aspect ratio. The nanoparticle also comprises at least one conductive shell surrounding said prolate spheroid-shaped core. The nanoparticle has a surface plasmon resonance sensitivity of at least 600 nm RIU(sup.-1). Methods of making the disclosed nanorice particles are also described herein

The [Ne III]/[Ne II] line ratio in NGC 253

We present results of the mapping of the nucleus of the starburst galaxy NGC 253 and its immediate surroundings using the Infrared Spectrograph on board the Spitzer Space Telescope. The map is centered on the nucleus of the galaxy and spans the inner 800 × 688 pc^2. We perform a brief investigation of the implications of these measurement on the properties of the star formation in this region using theories developed to explain the deficiency of massive stars in starbursts

The first Infrared study of the close environment of a long Gamma-Ray Burst

We present a characterization of the close environment of GRB980425 based on 5-160mic spectro-imaging obtained with Spitzer. The Gamma-Ray Burst GRB980425 occurred in a nearby (z=0.0085) SBc-type dwarf galaxy, at a projected distance of 900pc from an HII region with strong signatures of Wolf-Rayet (WR) stars. While this "WR region" produces less than 5% of the B-band emission of the host, we find that it is responsible for 45+/-10% of the total infrared luminosity, with a maximum contribution reaching 75% at 25-30mic. This atypical property is rarely observed among morphologically-relaxed dwarves, suggesting a strong causal link with the GRB event. The luminosity of the WR region (L_8-1000mic=4.6x10^8 Lsol), the peak of its spectral energy distribution at <~100mic and the presence of highly-ionized emission lines (e.g., [NeIII]) also reveal extremely young (<5Myr) star-forming activity, with a typical time-scale of only 47Myr to double the stellar mass already built. Finally, the mid-IR over B-band luminosity ratio in this region is substantially higher than in star-forming galaxies with similar L_IR, but it is lower than in young dust-enshrouded stellar clusters. Considering the modest obscuration measured from the silicate features (tau_9.7mic ~ 0.015), this suggests that the WR region is dominated by one or several star clusters that have either partly escaped or cleared out their parent molecular cloud. Combined with the properties characterizing the whole population of GRB hosts, our results reinforce the idea that long GRBs mostly happen within or in the vicinity of relatively unobscured galactic regions harboring very recent star formation.Comment: ApJ in press, 14 pages, 2 tables, 7 figure

Analysis of Heating Effects and Deformations for a STAF Panel with a Coupled CFD and FEM Simulation Method

Conventional sandwich panels are one of the cheapest and easiest solutions for forming the thermal building envelope of industrial buildings. They are pre-fabricated façade elements, of which millions of square metres have been produced and mounted every year. There is great potential to reduce the consumption of fossil fuels and CO2 emissions through the solar thermal activation of such a sandwich panel. In the course of the research project ABS-Network SIAT 125, a Solar Thermal Activated Façade (STAF) panel was designed which is to be optimised both thermally and structurally. This study shows a first version of a so-called ‘one way coupled’ thermal and structural analysis of a conventional sandwich panel compared to the STAF panel. For this purpose, the numerical methods of Computational Fluid Dynamics (CFD) and Finite Element Method (FEM) are used  together in one simulation environment. Furthermore, results from an outdoor test facility are presented where a first version of a STAF panel is tested under real climate conditions. The CFD model was positively evaluated by comparing measured and computed temperatures

Formation of escherichia coli O157: H7 Persister Cells in the Lettuce Phyllosphere and Application of Differential Equation Models to Predict Their Prevalence on Lettuce Plants in the Field

American Society for Microbiology. Escherichia coli O157:H7 (EcO157) infections have been recurrently associated with produce. The physiological state of EcO157 cells surviving the many stresses encountered on plants is poorly understood. EcO157 populations on plants in the field generally follow a biphasic decay in which small subpopulations survive over longer periods of time. We hypothesized that these subpopulations include persister cells, known as cells in a transient dormant state that arise through phenotypic variation in a clonal population. Using three experimental regimes (with growing, stationary at carrying capacity, and decaying populations), we measured the persister cell fractions in culturable EcO157 populations after inoculation onto lettuce plants in the laboratory. The greatest average persister cell fractions on the leaves within each regime were 0.015, 0.095, and 0.221%, respectively. The declining EcO157 populations on plants incubated under dry conditions showed the largest increase in the persister fraction (46.9-fold). Differential equation models were built to describe the average temporal dynamics of EcO157 normal and persister cell populations after inoculation onto plants maintained under low relative humidity, resulting in switch rates from a normal cell to a persister cell of 7.7×10-6 to 2.8×10-5 h-1. Applying our model equations from the decay regime, we estimated model parameters for four published field trials of EcO157 survival on lettuce and obtained switch rates similar to those obtained in our study. Hence, our model has relevance to the survival of this human pathogen on lettuce plants in the field. Given the low metabolic state of persister cells, which may protect them from sanitization treatments, these cells are important to consider in the microbial decontamination of produce. Despite causing outbreaks of foodborne illness linked to lettuce consumption, E. coli O157:H7 (EcO157) declines rapidly when applied onto plants in the field, and few cells survive over prolonged periods of time. We hypothesized that these cells are persisters, which are in a dormant state and which arise naturally in bacterial populations. When lettuce plants were inoculated with EcO157 in the laboratory, the greatest persister fraction in the population was observed during population decline on dry leaf surfaces. Using mathematical modeling, we calculated the switch rate from an EcO157 normal to persister cell on dry lettuce plants based on our laboratory data. The model was applied to published studies in which lettuce was inoculated with EcO157 in the field, and switch rates similar to those obtained in our study were obtained. Our results contribute important new knowledge about the physiology of this virulent pathogen on plants to be considered to enhance produce safety

Average Infrared Galaxy Spectra From Spitzer Flux Limited Samples

The mid-infrared spectroscopic analysis of a flux-limited sample of galaxies with fv(24um) > 10 mJy is presented. Sources observed are taken from the Spitzer First Look Survey (FLS) catalog and from the NOAO Deep Wide-Field Survey region in Bootes (NDWFS). The spectroscopic sample includes 60 of the 100 sources in these combined catalogs having fv(24um) > 10 mJy. New spectra from the Spitzer Infrared Spectrograph are presented for 25 FLS sources and for 11 Bootes AGN; these are combined with 24 Bootes starburst galaxies previously published to determine the distribution of mid-infrared spectral characteristics for the total 10 mJy sample. Sources have 0.01 < z < 2.4 and 41.8 < log vLv(15um) < 46.2 (ergs/s). Average spectra are determined as a function of luminosity; lower luminosity sources (log vLv(15um) < 44.0) are dominated by PAH features and higher luminosity sources (log vLv(15um) > 44.0) are dominated by silicate absorption or emission. We find that a rest frame equivalent width of 0.4um for the 6.2um PAH emission feature provides a well defined division between lower luminosity, "pure" starbursts and higher luminosity AGN or composite sources. Using the average spectra, fluxes fv(24um) which would be observed with the Spitzer MIPS are predicted as a function of redshift for sources with luminosities that correspond to the average spectra. AGN identical to those in this 10 mJy sample could be seen to z = 3 with fv(24um) > 1 mJy, but starbursts fall to fv(24um) < 1 mJy by z ~ 0.5. This indicates that substantial luminosity evolution of starbursts is required to explain the numerous starbursts found in other IRS results having fv(24um) > 1 mJy and z ~ 2.Comment: Accepted for publication in The Astrophysical Journa

Mechanical Stabilization of Helical Chirality in a Macrocyclic Oligothiophene

We introduce a design principle to stabilize helically chiral structures from an achiral tetrasubstituted [2.2]paracyclophane by integrating it into a macrocycle. The [2.2]paracyclophane introduces a three-dimensional perturbation into a nearly planar macrocyclic oligothiophene. The resulting helical structure is stabilized by two bulky substituents installed on the [2.2]paracyclophane unit. The increased enantiomerization barrier enabled the separation of both enantiomers. The synthesis of the target helical macrocycle 1 involves a sequence of halogenation and cross-coupling steps and a high-dilution strategy to close the macrocycle. Substituents tuning the energy of the enantiomerization process can be introduced in the last steps of the synthesis. The chiral target compound 1 was fully characterized by NMR spectroscopy and mass spectrometry. The absolute configurations of the isolated enantiomers were assigned by comparing the data of circular dichroism spectroscopy with TD-DFT calculations. The enantiomerization dynamics was studied by dynamic HPLC and variable-temperature 2D exchange spectroscopy and supported by quantum-chemical calculations